APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.5μg/ml |
| 0.5-1μg/ml |
| 0.5-1μg/ml |
|
Note :
IHC-P
Antigen retireval by heat
|
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
0.2mg Na₂HPO₄, 0.9mg NaCl, 5mg BSA
Preservative
0.05mg sodium azide, 0.05mg Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Annexin V (88-102aa SRLYDAYELKHALKG), identical to the related rat and mouse sequences.
Purification
Purified IgG
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
annexin A5 , PP4 , ANX5 , ENX2 , CPB-I , RPRGL3 , HEL-S-7 , VAC-alph
Cellular Localization
cytosol , endothelial microparticle , extracellular exosome , extracellular region , external side of plasma membrane , collagen-containing extracellular matrix , cytoplasm , focal adhesion , Membrane
Background
The Annexin 5 gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. Polymorphisms in this gene have been implicated in various obstetric complications. [provided by RefSeq, Dec 2019]
Database
Research Area
DATA IMAGES
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GTX03658 WB Image
WB analysis of various sample lysates using GTX03658 Annexin V antibody.
Lane 1 : Rat Brain Tissue Lysate at 50ug
Lane 2 : Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3 : Rat Ovary Tissue Lysate at 50ug
Lane 4 : Rat Lung Tissue Lysate at 50ug
Lane 5 : MCF-7 Whole Cell Lysate at 40ug
Lane 6 : SMMC Whole Cell Lysate at 40ug
Lane 7 : A549 Whole Cell Lysate at 40ug
Lane 8 : JURKAT Whole Cell Lysate at 40ug
Lane 9 : SGC Whole Cell Lysate at 40ug
Lane 10 : HT1080 Whole Cell Lysate at 40ug
Dilution : 0.5ug/ml
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GTX03658 ICC/IF Image
ICC/IF analysis of HCT116 cells using GTX03658 Annexin V antibody.
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GTX03658 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX03658 Annexin V antibody.
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GTX03658 IHC-P Image
IHC-P analysis of rat liver tissue using GTX03658 Annexin V antibody.
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REFERENCE
There are currently no references for Annexin V antibody (GTX03658). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Annexin V antibody (GTX03658). Be the first to share your experience with this product.
